Sample Group Research Articles

Molecular diagnosis and characterization of Anaplasma marginale and Ehrlichia ruminantium infecting beef cattle of Maputo Province, Mozambique

BackgroundMembers of the Anaplasmataceae family, such as the Anaplasma and Ehrlichia species, cause economic losses and public health risks. However, the exact economic impact has not been comprehensively assessed in Mozambique due to limited data available on its basic epidemiology. Therefore, we investigated the molecular occurrence and identity of Anaplasma and Ehrlichia spp. infecting beef cattle in Maputo province, Mozambique.MethodsA total of 200 whole blood samples were collected from apparently healthy beef cattle. Whole blood DNA was extracted and tested for presence of Anaplasma spp. and Ehrlichia ruminantium DNA through amplification of the 16S rRNA and map1 genes. Positive samples to Anaplasma spp. were subject to PCR assay targeting the A. marginale-msp5 gene. Amplicons obtained were purified, sequenced and subject to phylogenetic analyses.ResultsAnaplasma spp., A. marginale and E. ruminantium were detected in 153 (76.5%), 142 (71%) and 19 (9.5%) of all the samples analyzed, respectively. On this same sample group, 19 (9.5%) were co-infected with A. marginale and E. ruminantium. The 16S rRNA sequences of Anaplasma spp. obtained were phylogenetically related to A. marginale, A. centrale and A. platys. Phylogenetic analysis revealed that A. marginale-msp5 nucleotide sequences were grouped with sequences from Asia, Africa and Latin America, whereas E. ruminantium-map1 DNA nucleotide sequences were positioned in multiple clusters.ConclusionCattle in Maputo Province are reservoirs for multiple Anaplasma species. A high positivity rate of infection by A. marginale was observed, as well as high genetic diversity of E. ruminantium. Furthermore, five new genotypes of E. ruminantium-map1 were identified.

Is bone marrow biopsy and aspiration still mandatory when 18F-FDG PET/CT is available for the initial assessment of bone marrow metastasis in pediatric Ewing sarcoma?

PurposeTo compare the diagnostic value of 18F-fluorodeoxyglucose-positron emission tomography/computed tomography (18F-FDG PET/CT) and bone marrow biopsy and aspiration (BMBA) for evaluating bone marrow metastases (BMM) in newly diagnosed pediatric Ewing sarcoma (ES).Material and methodsTo assess the diagnostic accuracy of 18F-FDG PET/CT against BMBA for marrow infiltration in ES patients, a retrospective analysis encompassed 103 ES patients from the Children’s Hospital of Chongqing Medical University, spanning nine years, who underwent both 18F-FDG PET/CT and BMBA at the point of diagnosis.ResultsThe median age of this study was 9.3(15 days to 17.1 years), 52(50.5%) patients were male. Among the cohort, 8 subjects received a BMM diagnosis via marrow cytology or histopathology, concomitant with positive 18F-FDG PET/CT findings. An additional 4 patients were identified with BMM solely through 18F-FDG PET/CT. No cytologically or histologically positive BMM were found in PET/CT-negative patients. Therefore, within this selected sample group, the 18F-FDG PET/CT imaging technique exhibited sensitivity of 100% and specificity of 95.8%. The five-year overall survival rate decreased from 57.5% among the entire cohort of patients to a mere 30% for individuals suffering from BMM.ConclusionGiven these findings, the prevailing reliance on BMBA warrants reevaluation when 18F-FDG PET/CT is available, potentially heralding a shift towards less invasive diagnostic modalities in the management of ES.

Combining Game-Based Learning with Design Thinking Using Block-based Programming to Enhance Computational Thinking and Creative Game for Primary Students

This research aims to develop a combining game-based learning with design thinking using block-based programming to enhance computational thinking and creative games for primary students and will be referred to as game-based learning from now on. The purpose of this research is to 1) develop a model for game-based learning, 2) develop the system for game-based learning, 3) evaluate students' computational thinking after implementing game-based learning, and 4) evaluate the creative games created by students after game-based learning is implemented. The research tools included 1) the model for the game-based learning, 2) the model-appropriate evaluation form, 3) the learning system evaluation form 4) the computational thinking evaluation form, and 5) the creative game evaluation form. The research results showed that 1) the evaluation results of the model are appropriate for teaching at the highest level (x  = 4.82, SD = 0.42) and could be used for experimental teaching, 2) The results of game-based learning system quality are at the highest level (x  = 4. 57, S.D. = 0. 50). The researcher implements a game-based learning model and system to teach a sample group of 24 students in grade 4, using a purposive sampling method. The results of the implementation could be summarized as follows: 1) the results of students' computational thinking evaluation after implementing the model and system are significantly higher than before at the .05 level. 2) evaluation results of creative games that students developed after implementing the model and system are at a high level (x  = 4.29, S.D. = 0.52)

Item Analysis of Sociocultural Influences in Verbal Ability Testing Using the Woodcock-Johnson II COG

The article presents the results of a study that examines the impact of sociocultural environment on the development of verbal skills in Romani children aged 7–11 years, with a particular emphasis on children from socially excluded localities. The results are based on a verbal test derived from the Woodcock-Johnson Cognitive Ability Test (WJ IV COG), which was processed using item analysis according to the Rasch model. The main results are based on analysing the differential function of individual items within the verbal tests within the research group (N = 399) compared to the normative sample of the Czech population (N = 936). A secondary goal is to compare Romani children originating from socially excluded areas (N = 204) with those from non-excluded areas (N = 195). Regarding item comparisons between the majority and sample groups, they reveal significant disparities in 14 items (70%) of the Synonyms subtest and 9 items (45%) of the Antonyms subtest. The results highlight the crucial role of a more limited vocabulary, particularly in formal linguistic expressions by Romani children. The presented words representing differently functioning items can also serve as a basis for linguistic analysis and can be applied in assessing educational needs. When comparing the group of children based on their residence in a socially excluded locality, significant differences in the DIF were identified for one item from the Synonyms subtest and three items from the Antonyms subtest.

Assessment of reproductive outcomes of fresh versus cryopreserved ejaculated sperm samples-a retrospective analysis of 44423 oocyte donation ICSI cycles.

Does the use of frozen sperm affect live birth rate (LBR) and cumulative LBR (CLBR) compared to fresh sperm samples in oocyte donation ICSI cycles? Although there were slight decreases in pregnancy rates (PRs) and LBR, as well as CLBR per embryo replaced and per embryo transfer (ET), when frozen sperm samples were used compared to fresh ejaculates, their clinical impact was limited. Sperm cryopreservation is part of the daily routine in reproduction clinics worldwide because of its many advantages in cycle planning. Nonetheless, there is a lack of agreement in terms of its impact on the outcomes of ICSI cycles. Previous studies showed conflicting conclusions and focused on different populations, which makes reaching consensus on the impact of sperm freezing-thawing complicated. Moreover, classical parameters are used to assess cycle success: pregnancy, live birth and miscarriage rates per ET. This study reports those measurements plus CLBR, which more accurately reflects the impact of the technique on the likelihood of achieving a newborn. A retrospective multicenter observational cohort study, including data from 37041 couples and 44423 ICSI procedures from January 2008 to June 2022, was carried out. The group using frozen sperm included 23852 transferred embryos and 108661 inseminated oocytes, whereas the fresh sample group comprised 73953 embryos replaced and 381509 injected oocytes. Outcomes measured per first ET and per ET were compared between groups using Fisher's exact test and Chi-squared test, as appropriate. Binary-logistics regression models were used to adjust the analyses according to clinically relevant co-variables. Kaplan-Meier curves plotted the CLBR per oocyte inseminated, per embryo replaced and per ET, and compared between groups using the Mantel-Cox test. Cox regressions were employed for the multivariate analyses of CLBR. The frozen sperm group showed a slightly lower biochemical (3.55% and 2.56%), clinical (3.68% and 3.54%) and ongoing (3.63% and 3.15%) PR compared to the cycles using fresh sperm, respectively, both per first ET and per ET. LBR was 4.57% lower per first ET and 3.95% lower per ET in the frozen sperm group than the fresh sperm group. There was also a subtle increase of 2.66% in biochemical miscarriage rate per ET when using frozen versus fresh sperm. All these differences remained statistically significant after the multivariate analysis (adjusted P ≤ 0.001). There were statistically significant differences in CLBR per embryo replaced and per ET but not per oocyte used (adjusted P = 0.071). Despite the statistical significance of the differences between the groups, those using frozen sperm required only 0.54 more oocytes injected, 0.45 more embryos transferred and 0.41 more ET procedures, on average, to achieve a live birth compared to the fresh samples. The retrospective nature of the study subjects the data to biases or potential errors during annotation on the source clinical and cycle records. This study uses multivariate analyses to control biases as much as possible. Using the oocyte donation model also contributes to reducing heterogeneity in the oocyte quality factor. The large sample sizes included in this study allowed for the detection of small changes in cycle success rates between groups. Although statistically significant, the decrease in PRs, LBR, and CLBR when using frozen sperm can be clinically overlooked in favor of the many benefits of sperm cryopreservation. None declared. Not applicable.

Experimental study on the mechanism of coal spontaneous combustion inhibition by degradation of white-rot fungus

To investigate the mechanism of inhibition of spontaneous combustion of coal by degradation of white-rot fungus, the changes of macroscopic oxidizing characteristics and microstructure of the degraded coal were investigated by the programmed temperature-raising test and the in situ Fourier-transform infrared spectroscopy experiments. The difference in gas production between the degraded coal and the blank samples indicated that the degradation inhibited the oxidation process of coal, and the intensity of the inhibition effect was different at different oxidation stages. The strongest inhibition effect was found in the accelerated oxidation stage, i.e. 80–140 °C. To further analyze the micro-mechanism of the degradation of Phanerochaete chrysosporium affecting the spontaneous combustion of coal, the relative contents of active functional groups in coal samples were compared. The results showed that the degradation affected the relative contents of hydroxyl, aliphatic hydrocarbon, and oxygenated functional groups in coal. Among them, the most significant changes of –OH and –CO- were observed at 80–140 °C. Thus, it was analyzed that Phanerochaete chrysosporium could degrade the macromolecular structure of coal by generating biological enzymes to reduce the number of oxidizable functional groups such as –OH, –CH2, –CH3, –CO-, and so on, thus reducing the risk of spontaneous combustion of coal.

Rumours. Who believes them?

Purpose While psychology, sociology and communications studies hypothesise a range of independent variables that might impact on individuals’ acceptance or rejection of rumours, almost all studies of the phenomenon have taken place in environments featuring notable, and sometimes very deep, partisan divisions, making it almost impossible to isolate the impact of partisan influences on views on different rumour subjects. This study aims to remove the possibility of partisan influences on readers of internet rumours by testing the impact of independent demographic variables in China, a one-party state with no overt partisan divisions. The study provides an opportunity to strip away the influence of ideology and see whether this factor may have coloured previous studies on susceptibility to believe rumours. Design/methodology/approach An empirical study was used to examine belief in false and true online rumours in a non-partisan environment. A large sample group was presented with rumours across four subject areas and respondents’ conclusions and demographic information was then subject to logistic regression analysis to identify relationships between factors and ability to identify the veracity of online rumours. Findings Unexpectedly, the regression analysis revealed no statistically significant nexus between many independent demographic variables and patterns of believing or disbelieving rumours. In other cases, a statistically significant relationship was revealed, but only to a limited degree. The results suggest that once the role of partisanship in explaining the proliferation of and belief in false rumours and the ability to identify true ones is removed from consideration, no other independent variables enjoy convincing links with rumour belief. Originality/value The study tests in China, a jurisdiction featuring a non-partisan environment, the impact of independent variables on media users’ belief in a wide range of rumours.

Evaluation of humoral and cellular immune responses against Vibrio cholerae using oral immunization by multi-epitope-phage-based vaccine

IntroductionCholera is a severe gastrointestinal disease that manifests with rapid onset of diarrhea, vomiting, and high mortality rates. Due to its widespread occurrence in impoverished communities with poor water sanitation, there is an urgent demand for a cost-effective and highly efficient vaccine. Multi-epitope vaccines containing dominant immunological epitopes and adjuvant compounds have demonstrated potential in boosting the immune response. Material and methodsB and T epitopes of OMPU, OMPW, TCPA, CTXA, and CTXB proteins were predicted using bioinformatics methods. Subsequently, highly antigenic multi-epitopes that are non-allergenic and non-toxic were synthesized. These multi-epitopes were then cloned into the pCOMB phagemid. A plasmid M13KO7ΔpIII containing all helper phage proteins except pIII was created to produce the recombinant phage. Female Balb/c mice were divided into three groups and immunized accordingly. The mice received the helper phage, recombinant phage or PBS via gavage feeding thrice within two weeks. Serum samples were collected before and after immunization for the ELISA test as well as evaluating immune system induction through ELISpot testing of spleen lymphocytes. ResultsThe titer of the recombinant phage was determined to be 1011 PFU/ml. The presence of the recombinant phage was confirmed through differences in optical density between sample and control groups in the ELISA phage technique, as well as by observing transduction activity, which demonstrated successful production of a recombinant phage displaying the Vibrio multi-epitope on M13 phage pIII. ELISA results revealed significant differences in phage antibodies before and after inoculation, particularly notable in the negative control mice. Mice treated with multi-epitope phages exhibited antibodies against Vibrio cholerae lysate. Additionally, ELISpot results indicated activation of cellular immunity in mice receiving both Vibrio and helper phage. ConclusionThis study emphasizes the potential of multi-epitope on phage to enhance both cellular and humoral immunity in mice, demonstrating how phages can be used as adjuvants to stimulate mucosal immunity and act as promising candidates for oral vaccination.

Facebook Challenge: Integration of Breastfeeding for Sick Babies and E-Training to Enhance the Knowledge of Healthcare Workers

Background Although the Thailand Breastfeeding Center has supported breastfeeding for sick babies since 2014, it has been found that breastfeeding in infants is still 40% lower than national targets. Objective This research aimed to test the effectiveness of online breastfeeding training through social media to further the knowledge of healthcare workers. Methods A quasi-experimental research study of a sample group of 60 workers was conducted between the neonatal step-down unit and the postpartum care unit. Research instruments consisted of breastfeeding e-training through a Facebook platform, knowledge testing on the breastfeeding of sick newborns, an online training satisfaction assessment form, data analysis by frequency and percentage, averaging, and testing by t-test, chi-square, and the Fisher exact test. Results The mean and standard deviation of knowledge before training in the experiment and control group were 22.40±2.11and 23.43±3.32, respectively. After the intervention, the experimental group was 31.93±1.98, and the control group was 24.06±2.14. It was found that the average knowledge score pertaining to the breastfeeding of high-risk neonates in the group that had undergone online training was higher than the group that had undergone ordinary supervision (p< 0.001). Conclusion It can be seen that asynchronous e-training may be one alternative for the knowledge development of healthcare workers in promoting breastfeeding for families with sick newborns.

A nuanced view of the extent to which samples from narrow populations are scientifically problematic.

Psychologists have a traditional concern with participant samples from narrow populations and deleterious effects on researchers' ability to generalize findings. Recently, both individuals and authoritative organizations, such as the American Psychological Association, have merged this external validity concern with diversity and inclusion concerns. The American Psychological Association directive for researchers to include diverse samples seems obviously well-taken as it purports to mitigate these problems at once; it simultaneously increases external validity and promotes diversity and inclusion. However, we show that there are complications. These include problems with internal and external validity conceptualizations; that sometimes generalization failures can support, rather than detract from, external validity; the crucial role auxiliary assumptions play in impacting internal and external validity; Lakatosian degenerative science and its problematic application; and distinguishing between merely including diverse groups in research samples versus analyzing for group differences. These complications imply a nuanced perspective of whether samples from narrow populations are undesirable. That a sample is from a narrow population might, or might not, preclude strong support or disconfirmation for the theory, including its ability to generalize. Our nuanced perspective militates against the current trend of journal directives to require diverse samples. Sample suitability for particular researcher goals should be judged on a case-by-case basis that takes into account that sometimes samples from narrow populations can nevertheless engender impressive scientific progress and sometimes not. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Lifetime Polyvictimization and Mental Health Outcomes in Women With vs Without Incarceration Histories: A Population-Based Latent Class Analysis.

We use latent class analysis, a life course framework, and information on the type, frequency, and timing of trauma exposure to identify distinct polytrauma groups in a national sample of women (AddHealth). We compare the identified polytrauma groups and their associations with mental health in adulthood in women with and without incarceration histories. A unique group with polyvictimization (neglect, physical, sexual) exposure in childhood by a caregiver in women with incarceration histories was not identified in women without incarceration histories. We find evidence of distinct associations between polytrauma groups and mental health and possibly, criminal justice involvement, in adulthood.

Development of a biodegradable polymer-based implant to release dual drugs for post-operative management of cataract surgery.

Cataract surgery is followed by post-operative eye drops for a duration of 4-6 weeks. The multitude of ocular barriers, coupled with the discomfort experienced by both the patient and their relatives in frequently administering eye drops, significantly undermines patient compliance, ultimately impeding the recovery of the patient. This study aimed to design and develop an ocular drug delivery system as an effort to achieve a drop-free post-operative care after cataract surgery. An implant was prepared containing a biodegradable polymer Poly-lactic-co-glycolic acid (PLGA), Dexamethasone (DEX) as an anti-inflammatory drug, and Moxifloxacin(MOX) as an antibiotic. Implant characterization and drug loading analysis were conducted. In vitro drug release profile showed that the release of the two drugs are correlated with the clinical prescription for post operative eye drops. In vivo study was conducted on New Zealand albino rabbits where one eye underwent cataract surgery, and the drug delivery implant was inserted into the capsular bag after placement of the synthetic intraocular lens (IOL). Borderline increase in the intraocular pressure (IOP) was noted in the test sample group. Slit-lamp observations revealed no significant anterior chamber reaction in all study groups. Histopathology study of the operated eye revealed no significant pathology in the test samples. This work aims at developing the intra ocular drug delivery implant which will replace the post-operative eye drops and help the patient with the post-operative hassle of eye drops.

Impact of Cultural Diversity on Employee Performance

The purpose of this study was to investigate the influence of cultural diversity on job satisfaction and its effect on expatriate work performance. Structured interviews were conducted to collect qualitative data from a sample group of expatriates five participants from mixed nationalities in a private sector organization based in the United Arab Emirates. Thematic analysis identified three emergent themes of culture that negatively impacted expatriates' job satisfaction, causing reduced employee performance, and two themes were identified that increased expatriate performance at work (enhanced creativity and innovation). This research contributes to the body of academic knowledge about the effect of cultural diversity on expatriate employee performance. The value for praxis from this study are recommendations for managerial action to improve expatriate work performance.

Comparative evaluation of remineralization potential of novel bioactive agents on eroded enamel lesions: A single-blinded in vitro study

Objective: The escalating prevalence of noncarious tooth wear stands as a critical concern in the backdrop of evolving lifestyles and dietary patterns. Dental erosion, a progressive condition induced by both endogenous and exogenous acidic influences, directly impacts enamel integrity, resulting in surface loss. The contemporary surge in carbonated beverage consumption further exacerbates this erosive milieu, underscoring the urgency for dental practitioners to adopt meticulous treatment strategies. Existing literature underscores a noteworthy 94% reduction in tooth erosion risk for individuals abstaining from sweetened soft beverages, emphasizing the imperative for a well-devised remineralization protocol to counter demineralized surfaces. Methodology: Seventy-three enamel specimens were taken. Forty samples were subjected to pre-operative hardness testing, and five samples were subjected to baseline EDX evaluation followed by grouping of samples (Group 1 = control Group; Group 2 = casein phosphopeptide–amorphous calcium phosphate fluoride [CPP-ACPF] Group; Group 3 = Biomin F Group; and Group 4 = self-assembling peptide [SAP] P-114 Group). A demineralization–remineralization cycle was carried out for 5 days followed by testing through Vickers Microhardness Tester, EDX Evaluation, and Scanning Electron Microscopy (SEM) Imaging. Statistical analysis was performed using one-way analysis of variance followed by intergroup analysis using Tukey’s post hoc test with SPSS software 25.0 version. Results: The mean percentage change in microhardness values was 30.05% in Group 1, 24.21% in Group 2, 18.85% in Group 3, and 12.08% in Group 4. The mean Ca/P ratio of samples tested through EDAX was 2.20 at baseline, 1.40 in Group 1 (Control Group), 1.62 in Group 2 (CPP-ACPF), 1.82 in Group 3 (Biomin F), and 2.01 in Group 4 (SAP-P114). Postintervention values were statistically significant from baseline values in both parameters. Conclusion: Curodont Protect exhibits superior efficacy, offering valuable insights for future in vivo studies and clinical applications. The multifaceted evaluation, encompassing microhardness testing, SEM analysis, and EDXS assessment, contributes to a nuanced interpretation of the agents’ impact, paving the way for informed decisions in clinical practice and future research endeavors.

Anti-Corruption, Credit Supply, and Agricultural Economic Development

This study analyzes regional panel data from China (2013-2019) to explore how the government's anti-corruption efforts and credit supply affect agricultural economic development. The empirical results show that anti-corruption and credit supply can effectively enhance agricultural economic development, and there is a complementary relationship between anti-corruption and credit supply, meaning that anti-corruption measures and credit supply can jointly promote the development of the agricultural economy. Further analysis indicates that the complementary and synergistic effect of the two on agricultural economic development is more pronounced in sample groups with a lower degree of marketization.

Improvements in the Application of the Tawarruq Munazzam Contract in Malaysian Islamic Banking: An Analysis from a Shariah Perspective

This paper aims to examine the measures taken by selected banks in Malaysia to enhance the implementation of tawarruq munazzam contracts. As a qualitative study, the researcher gathered data through library research and empirical studies. The library research involved analyzing books, articles, and statutes, while the field studies consisted of unstructured interviews with eight participants, including officers and Shariah committees of Islamic Finance Institutions (IFI). The sample group was selected using the purposive sampling method. Consequently, the researcher identified several themes from the interview data, which were subsequently analyzed. Tawarruq munazzam was chosen as a case study due to its prevalence as the most popular financial instrument among Islamic banks in Malaysia. The research findings revealed nine issues related to the tawarruq munazzam contract. Furthermore, the study highlighted the lack of uniformity among the five surveyed banks in terms of their efforts to improve the practice of tawarruq munazzam contracts. Additionally, one unresolved issue identified by the study pertained to the inclusion of wakalah elements. Notably, the study found that one of the banks exhibited the highest level of improvement in the practice of tawarruq munazzam, addressing eight out of the nine issues raised. 

Strategy to Empower Nontargeted Metabolomics by Triple-Dimensional Combinatorial Derivatization with MS-TDF Software.

Chemical derivatization is a widely employed strategy in metabolomics to enhance metabolite coverage by improving chromatographic behavior and increasing the ionization rates in mass spectroscopy (MS). However, derivatization might complicate MS data, posing challenges for data mining due to the lack of a corresponding benchmark database. To address this issue, we developed a triple-dimensional combinatorial derivatization strategy for nontargeted metabolomics. This strategy utilizes three structurally similar derivatization reagents and is supported by MS-TDF software for accelerated data processing. Notably, simultaneous derivatization of specific metabolite functional groups in biological samples produced compounds with stable but distinct chromatographic retention times and mass numbers, facilitating discrimination by MS-TDF, an in-house MS data processing software. In this study, carbonyl analogues in human plasma were derivatized using a combination of three hydrazide-based derivatization reagents: 2-hydrazinopyridine, 2-hydrazino-5-methylpyridine, and 2-hydrazino-5-cyanopyridine (6-hydrazinonicotinonitrile). This approach was applied to identify potential carbonyl biomarkers in lung cancer. Analysis and validation of human plasma samples demonstrated that our strategy improved the recognition accuracy of metabolites and reduced the risk of false positives, providing a useful method for nontargeted metabolomics studies. The MATLAB code for MS-TDF is available on GitHub at https://github.com/CaixiaYuan/MS-TDF.

Levels of teacher performance in formative assessment in multigrade and single-grade classrooms

Formative assessment is an evaluative practice developed in the classroom for the improvement of learning using evidence on student progression. The objective of this research is to compare sample groups from multigrade and single-grade classrooms on the theme of formative assessment based on the students' opinion of the teacher's performance. The method used was a comparative quantitative method. The sample type is a probability sample of 683 students from 5th to 8th grade from urban and rural schools in the commune of Longaví, located in the Maule Region of Chile. A validated Likert scale questionnaire with a high level of reliability (α = 0.93) was used. The results of the research showed that, in the six dimensions, the best teacher performance concerning formative assessment is found in multi-grade schools and not in single-grade schools. This can be explained on the basis of several reasons, among them the level of adaptability that teachers have in this type of classroom, the heterogeneous characteristics of the classroom (different ages and learning goals) and the need for teachers to monitor the learning progression of students with different classroom characteristics.

Milling degree affects the fermentation properties of rice: perspectives from the composition of nutrients and gut microbiota via in vitro fermentation

Fermentation substrates of rice with different milling degrees (MDs) were prepared and fermented with human feces to compare their fermentation properties and effects on gut microbiota. MD 0s, MD 5s and MD 60s represented brown rice, moderately-milled rice and white rice, respectively. After in vitro fermentation, the MD 5s group showed higher starch utilization, compared with the MD 0s and 60s groups evaluated by Fourier transform infrared spectrometer, and confocal laser scanning microscope. Effects of fermentation substrates of rice with different MDs on gut microbiota were evaluated by 16S rDNA sequencing. All the sample groups reduced the pH and produced short-chain fatty acids (SCFAs) and branched-chain fatty acids. The MD 5s group exhibited higher α-diversity than the MD 0s and 60s groups. Abundances of Phascolarctobacterium, Blautia and norank_f_Ruminococcaceae were higher in the MD 0s and 5s groups, compared with the MD 60s group. These bacteria were also positively correlated with the SCFAs production via Spearman correlation analysis. In vitro culture assay revealed that fermentation substrates of MD 0s and 5s promoted the growth of two probiotics (Akkermansia muciniphila and Bifidobacterium adolescentis). Our results showed that moderate milling might be an appropriate way to produce rice products with richer nutrients and better fermentation properties.

Study of the Level of Sports Addiction Among Students at the Faculty of Sports Science

The aim of this study was to investigate the exercise addiction of the students of the School of Physical Education and Sport and to determine the differences according to the type of exercise, age of exercise and load dynamics. The Exercise Addiction Scale (EBÖ-21) developed by Housenblas and Downs (2002) and the Turkish Validity and Reliability Study by Yeltepe and İkizler (2007) and the Personal Information Form were applied to the sample group. The sample of the study consisted of 245 athletes who regularly train at Afyon Kocatepe University School of Physical Education and Sports. Studies on this subject indicate that addiction to sports is similar to addiction to chemical substances due to the presence of withdrawal and tolerance symptoms. The results of the study presented show that self-confidence and athletic performance increase as the students' level increases. When evaluating exercise addiction by gender, it was found that female students were more likely than male students to consider physical problems and show self-control and interruption of exercise behaviour. When evaluated by age, exercise addiction was found to have decreased and been brought under control in older individuals, indicating progress in self-control of addiction.